This calculation assumes the average mass of an RNA nucleotide is 340 Daltons (Da), the mass of a DNA nucleotide is 330 Da and the mass of a basepair (bp) in double stranded DNA is

660 Da. This makes the formula weight

^{*} of RNA nucleotides

^{*} 340 g, the formula weight of DNA nucleotides 300 g and the formula weight of the bp's in double stranded DNA

660 g.

Using these numbers, the formula weight of any single or double strand of nucleotides can be estimated by taking the product of the strand length (in bases or bp's) and the average formula weight of the type of nucleotide in the strand.

The inverse of the formula weight is the number of moles of template present in one gram of material.

Using Avogadro's number, 6.022x10

^{23} molecules/mole, the number of molecules of the template per gram can be calculated:

Finally, the number of molecules or number of copies of template in the sample can be estimated by multiplying by 1*10

^{9} to convert to ng and then multiplying by the amount of template (in ng)

This calculator requires the user to input the amount of a template present (in ngs) and the length of the template (in bases or bp's) and with this information the number of copies of the template is calculated.

The formula used is:

## Comments

## Perfect, bookmarked! Could I

Perfect, bookmarked! Could I have one for RNA as well?

## Done

The calculator now does ds and ssDNA* and ssRNA

## Thanks

Thank you ....this is so helpful.

## You are welcome

I am glad you found it useful

## Thanxxxxxxxxxxxxxxxxxxxxxxxxx

Thanxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

## RNA genomes

For ssRNA, Does this formula consider 2.53x10E6 g/mol as the molecular size for genomic RNA?

## Not sure what you are asking

This calculator converts from ng to number of copies based on template length. Is this what you are trying to do?

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