This calculation assumes the average mass of an RNA nucleotide is 340 Daltons (Da), the mass of a DNA nucleotide is 330 Da and the mass of a basepair (bp) in double stranded DNA is 660
Da. This makes the formula weight*
of RNA nucleotides*
340 g, the formula weight of DNA nucleotides 300 g and the formula weight of the bp's in double stranded DNA 660
Using these numbers, the formula weight of any single or double strand of nucleotides can be estimated by taking the product of the strand length (in bases or bp's) and the average formula weight of the type of nucleotide in the strand.
The inverse of the formula weight is the number of moles of template present in one gram of material.
Using Avogadro's number, 6.022x1023
molecules/mole, the number of molecules of the template per gram can be calculated:
Finally, the number of molecules or number of copies of template in the sample can be estimated by multiplying by 1*109
to convert to ng and then multiplying by the amount of template (in ng)
This calculator requires the user to input the amount of a template present (in ngs) and the length of the template (in bases or bp's) and with this information the number of copies of the template is calculated.
The formula used is: