# Punnett Square Calculator

A Punnett Square* shows the genotype*s two individuals can produce when crossed. To draw a square, write all possible allele* combinations one parent can contribute to its gametes across the top of a box and all possible allele combinations from the other parent down the left side. The allele combinations along the top and sides become labels for rows and columns within the square. Complete the genotypes in the square by filling it in with the alleles from each parent. Since all allele combinations are equally likely to occur, a Punnett Square predicts the probability of a cross producing each genotype.

Number of traits in cross:

Cross:
Edit Alleles:
Parent 1:

Trait 1
Trait 2
Trait 3
Trait 4
Trait 5

Parent 2:

Show:
Phenotype
Genotype

Percent
Ratio

A single trait Punnett Square tracks two alleles for each parent. The square has two rows and two columns. Adding more traits increases the size of the Punnett Square. Assuming that all traits exhibit independent assortment, the number of allele combinations an individual can produce is two raised to the power of the number of traits. For two traits, an individual can produce 4 allele combinations (2^2). Three traits produce 8 combinations (2^3). Independent assortment typically means the genes are on different chromosome*s. If the genes for the two traits are on the same chromosome, alleles for each trait will always appear in the same combinations (ignoring recombination).

With one row or column for each allele combination, the total number of boxes in a Punnett Square equals the number of rows times the number of columns. Multi-trait Punnett Squares are large. A three trait square has 64 boxes. A four trait square has 256 boxes.

The genotype in each box is equally likely to be produced from a cross. A two-trait Punnett Square has 16 boxes. The probability of a cross producing a genotype in any box is 1 in 16. If the same genotype is present in two boxes, its probability of occurring doubles to 1/8 (1/16 + 1/16).

If one of the parents is a homozygote for one or more traits, the Punnett Square still contains the same number of boxes, but the total number of unique allele combinations is 2 raised to the power of the number of traits for which the parent is heterozygous.

A commonly discussed Punnett Square is the dihybrid cross. A dihybrid cross tracks two traits. Both parents are heterozygous, and one allele for each trait exhibits complete dominance*. This means that both parents have recessive alleles, but exhibit the dominant phenotype. The phenotype ratio predicted for dihybrid cross is 9:3:3:1. Of the sixteen possible allele combinations:

• Nine combinations produce offspring with both dominant phenotypes.
• Three combinations each produce offspring with one dominant and one recessive phenotype.
• One combination produces a double recessive offspring.
• This pattern only occurs when both traits have a dominant allele. With no dominant alleles, more phenotypes are possible, and the phenotype probabilities match the genotype probabilities.

A simpler pattern arises when one of the parents is homozygous for all traits. In this case, the alleles contributed by the heterozygous parent drives all of the variability. A two trait cross between a heterozygous and a homozygous individual generates four phenotypes, each of which are equally likely to occur.

More complicated patterns can be examined. In an extreme case when more than two alleles exists for each trait and the parents do not possess same alleles, the total number of genotypes equals the number of boxes in the Punnett Square.

It is possible to generate Punnett squares for more that two traits, but they are difficult to draw and interpret. A Punnett Square for a tetrahybrid cross contains 256 boxes with 16 phenotypes and 81 genotypes. A third allele for any one of the traits increases the number of genotypes from 81 to 108.

Given this complexity, Punnett Squares are not the best method for calculating genotype and phenotype ratios for crosses involving more than one trait.

Test your understanding with the Punnett Square Calculator Problem Set.

Video Overview

Related Content

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### Love how you can do many

Love how you can do many traits! Captures what it aims to do really good.

...and it runs on linux!!!

### It also runs on

windows, mac, android and iOS.

I even tried it on my daughter's DSi, but the DSi ran out of memory before the page loaded completely.

### Question

In the last seconds of the video the narrator says:

“Given this complexity, Punnett Square It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. "> It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. " class="lexicon-term">*s are not the best method for calculating genotype* and phenotype* ratios for crosses involving more than one trait.”

Okay, what is the best method?

Because of the sheer number of allele* combinations possible when tracking multiple, independently assorting traits, we usually don't calculate the probability for all genotype*s.  It is more common to focus on a small set of possible genotypes. For example, you might be asked to calculate the probability that a specific paring will result in offspring containing one or more recessive traits. In this case, we can ignore all but a few genotypes and avoid generating a huge Punnett Square It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. "> It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. " class="lexicon-term">*.

Instead, we calculate the probability for each trait individually using single trait Punnett Squares. Then, given independent assortment, we can multiply the individual probabilities together to find the probability of producing specific multi-trait combinations.

Consider a tryhibrid cross AaBbCb x AaBbCc. If we want to know the probability of producing a triple heterozygous offspring (AaBbCc). We first treat each trait separately and determine that there is a 50% (0.5) chance that an offspring will be heterozygous for each of the traits A, B and C.

The probability that an offspring will be heterozygous for all three traits is the product of the individual probabilities: 0.5 x 0.5 x 0.5 = 0.125. There is a 12.5% chance that the mating will produce a triple heterozygous offspring.

Once we know all of the single trait probabilities, it is easy to calculate additional multi-trait probabilities. The probability of the cross above (AaBbCc x AaBbCc) producing AAbbCc is:

(0.25 for AA) times (0.25 for bb) times (0.5 for Cc) = 0.25 x 0.25 x 0.5 =  0.03125 or 3.125%

The key thing to grasp is that independent assortment allows us to multiply individual trait probabilities to find multi-trait probabilities.

If for some reason you want to calculate the probability for every genotype in a multi-trait cross, it is still better to calculate them by multiplying the single trait probabilities. Because, it is easy to make mistakes when drawing large Punnett Squares.

There is a third technique involving drawing branching diagrams, but I don't use it.

### Thank you.  That was very

Thank you.  That was very helpful.

### Error in 5x5 square computation?

Either I am reading this wrong, or there is an error in the program when you take it up to 5x5.

Reading across the top row there when you do a cross of  AaBbCcDdEe × AaBbCcDdEe I am only seeing 30 uinque entries across the top.  It appears that ABcDe and aBcDe are missing and replaced by duplications of other combinations.

### Fixed

You were right, there was a typo in an aray.  What did I say about it being easy to make mistakes drawing such large squares?

### Quite true

Your point is driven home.

### AP Biology

I'm trying to do my homework and I don't have the time to do the punnett square It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. "> It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. " class="lexicon-term">*s.

### Phenotype ratio error

The Punnett Square It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. "> It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. " class="lexicon-term">* display for Phenotype* Ratio/% is incorrect if we are showing Mendelian (Dominant/Recessive) genetics*.  For example:  Aa x Aa cross would produce a Genotype* of 25% AA : 50% Aa : 25% aa,  but the Phenotype would be 75% "A"(AA+Aa) : 25% "a" (aa).

### Can't reproduce

@Gmager: Thanks for the feedback. I am unable to reproduce your error. If I set the number of traits to 1, have two heterozygous (Aa) parents, make either allele* (A or a) the dominant allele and show Phenotype* percent or ratio, I get two distinct phenotypes with the appropriate frequencies.

If the radio button for dominant allele is left at the default setting of "None", the calculator assumes that Aa produces an intermediate phenotype. Under these conditions the phenotype probability matches the genotype* probability (25:50:25)

### SCIENCE

HOW DO I DO THE PUNNETT SQUEAR..THANK YOU AND HAVE A GREAT DAY SINCERLY BARONNA FREEMAN

### The way it works is like a

The way it works is like a multiplication table. I hope i answered your question.

### This saved my life!!!! thank

This saved my life!!!! thank you!

### Calculating Phenotypic Raitios for multiple traites

Is there another way to determine Phenotypic ratios without using a punnett square. I know how to calculate the number of traite categories but not the ratios in each.

### Calculating individual phenotypic ratios

You can calculate phenotypic ratios using the process described for genotypic ratios in this comment.  Does this answer your question?

### Share code?

Would you consider sharing the code, or at least the algorithm, you use in calculating the results of the crosses? I'm trying to come up with something similar in R; but, rather than produce a Punnett Square It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. "> It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. " class="lexicon-term">*, it will feed the numerical results of extremely large numbers of loci into calculations following up on Fisher's (1918) polygenic model. I'm trying to avoid a brute force solution, but I'm embarassed I haven't found a simple algorithm yet!

Thanks in advance, and really nice work on this page!

### contact me

DGM - I will give you whatever help I can.  Send me a message using the site contact form: http://www.scienceprimer.com/contact

### Hmm

Your video go's way to fast. I don't know what kind of audience it's directed at, but I've done punnett square It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. "> It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. " class="lexicon-term">*s before and I can't follow anything you said. It's explaining how to do a math equation in four minutes.

### Overview

John, thanks for the input. There is quite a bit of information packed into the video. As the title states, it is an overview. If you want to gain a good understanding of the concepts covered, read over the material on this page, spend some time with the calculator and take a look at the links under "related content".

### Wonderful Program!

This is an excellent thing I didn't (unfortunately) know existed in my high school biology course! I use it all the time now for my college biology homework! :)

great

### Told the phenotype ratios are wrong by professor

Hi, I was told you cannot write phenotype*s in "GG" Gg" format.  Why are you expressing them this way?  I was told my ratios were completely wrong since my tutor told me to check my work here.  Now, I am told there is no way a phenotype is EVER expressed like that for a ratio. It was by a professor with their doctorate. Could you advise what you mean by your answers?  I got 25% of my grade wrong because I used the phentype ratio you suggest.   Thank you. I don't mind to mean unappreciative.

### Phenotypes labels must be translated for each specific case

Thank you for the comment. I am sorry you had trouble with your exam. Your professors rationale is that a genotype is the alleles present at the loci of interest and the phenotype is the physical expression of that genotype. By this reasoning, the labels for phenotype ratios should always be the description of the characteristic, rather than the allele patterns.

Since this calculator does not ask you to enter the traits for each gene locus, it is unable to label the ratios with the trait descriptions. Give this limitation, it uses allele patterns (each drawn in a distinct color) as proxies for the different phenotypes.

The phenotype ratios provided by the calculator are correct. But the phenotypes are labeled with allele patterns because the calculator does not know what trait is expressed by AA, gG or whatever other allele abbreviation you enter.

I could rewrite the calculator to show colored blocks instead of alleles. Unfortunately, I fear this would be trading one type of confusion for another because some of the colors would be difficult to tell apart, especially in the larger tables.

### Thank you

I see exactly what you are saying. Thank you for your kind comment. The problem lies in my not knowing how to express the phenotype* and not in your program.  Thank you again.

### Is it possible to generate a

Is it possible to generate a downloadable image file of the punnett square It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. "> It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. " class="lexicon-term">*?

### The square is text not an image

The easiest thing to do is to scale the square to the size you want and take a screen shot.

There is no easy alternative because the square is not rendered as an image. It is a set of text boxes arranged in rows and columns. If you know anything about html, each box is a div elelment with a colored background.

If you are comfortable going into the source, you can grab the content of the div with the id "squarePositionDiv" and paste it into another html file.

### So good!

Thanks for this! i'm from south america and with this make my biology homework more  easier... Sorry for my bad english! :) Graciasss!

### science

need a picture of squaresi

### calculator

would love codominance and/or incomplete

### Set dominant trait to none

Codominance and/or incomplete dominance* patterns can be shown with the calculator by setting the dominant allele* to 'none' for the incomplete and co-dominant traits.

### Confused

Isnt there supposed to be a diffrence between genotype*s and phenotype*s with linked genes? just sayin

### Calculator assumes independent assortment

@rick: I am not sure what you are asking. The patterns shown assume independent assortment. The calculator doesn't work for linked genes.

### Punnett square for ADD.

Punnett square It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. "> It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. " class="lexicon-term">* for ADD.

### What is this

I dont understand what it does though!

### I cant read the 5-trait cross

I cant read the 5-trait cross

### What are you trying to get?

You can see all of the genotype* or phenotype* frequencies by clicking the "show frequencies" button at the top

### Knowing how much Linux is

Knowing how much Linux is used in the science industry, it make me happy when websites provide interactive content that isn't platform dependant.

### Thanks

I try to be as platform neutral as possible. Of course, most of the site will not work with IE8... :-)

### Blood Type

is there a blood type punnet square calculator anywhere?

### Can use this calculator to get the genotypes

This calculator will give you the genotype*s for blood type crosses, but it can not deal with the codominance of the A and B allele*s. So, you'll have to calculate some of the phenotype* probablities on your own.

For example:

1. set the number of traits to cross to 1
2. give parent 1 the alleles A and O
3. give parent 2 the alleles A  and B

you get 4 genotypes:

AA, OA AB and OB. With AB codominance the pheotypes will be A, AB and B

Frequencies will be

A = AA + AO = 25% + 25% = 50%

B = OB = 25%

AB = AB = 25%

To make this work you need to remember that the genotypes AA and AO give the same phenotype as do BB and BO.

### Punnet square help!!

I have been trying to make a punnet squarre as slisted below.  Did it myself and was told it was wrong!  Used this calculater ans was told it was wrong.  Can anyone help!

Use a punnett square It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. "> It is most useful when looking at one or two loci because the number of possible genotypes increases rapidly as the number of loci observed increases. A single locus Punnett square contains four allele combinations. A two-locus Punnett Square contains 16 combinations, and a three locus square contains 64.
A pairing where two traits are observed is called a dihybrid cross.
Multi-locus Punnett Squares can be hard to draw and are difficult to interpret. The large number of genotypes possible with multi-locus crosses makes it easier to avoid drawing the square. Instead, the probability of specific genotypes arising are calculated using equations. " class="lexicon-term">* to figure out the results and type in the phenotype* ratios including the types (tall green or what ever is correct) for the following crosses,  a cross between the following parents TTyy  x TtYy

### Convert allele patterns to phenotype labels

Marie,

I am sorry you are having trouble. Are you converting the allele* labels from the phenotype* frequencies to the actual phenotypes descriptions?  See this comment from above for more context: http://scienceprimer.com/comment/937#comment-937

### blue eyes or light eyes over darker eyes

Why is it that on my fathers side there are brown or green or hazle eyes and brown or black hair one a red head. My father has hazle (more green than brown). My father was married twice in his first marrige his son has dark blue eyes and brown hair . His second marrige to my mother. I the first son have light blue eyes and blond hair and my younger brother has brown eyes and dark brown hair. What dose that mean? Both sides of the family  can be traced back. Dads side pure Polish and my mother British. Yet the children look different? I guess Its the amazement of genetics* but its still confusing and facinating.

### Finals + Punnet Squares = very stressed

Im attempting to do the punnet square and I understand the concept of it and everything but everytime I set it up I am unsure of whether I have the right set up which this has helped very much with! Although I have to include male and female genes and this seriously screws me up! How can you have a dominate male and a dominate female? I actually have to do this for part of my finals so I want to get it right. Can anyone help me!??!

### productores por un día

Se Realiza mutación una, any Cambio heredable en el material de hereditario (ADN) Afectan a Las Células Productoras De gametas apareciendo gametos estafadores Mutaciones. Se transmiten a la siguiente Generación Y Tiene Una alcaldesa importância desde el punto de vista evolutivo. Las Plantas de maíz pueden servi regeneradas del tanto porción organogénesis COMO porción embriogénesis somáticas pecado Que Exista Fusión de gametas, y dan Lugar a la Formación de Plantas Completas. Se USAN en el maíz PORQUE Producen facilmente callos embriogénicos. Teniendo el los genes recesivos señorea y.

GENOTIPOS ----- AA = 1 = 2 Aa aa = 3

Fenotipos ------ A = 3 a = 1

Si cruzamos dos Híbridos obtendremos Una Descendencia al juntar dos gametos:

1 - AABB 1aabb 1AAbb 1aaBB 2AABb 2AaBB 2Aabb 2aaBb 4AaBb

### I don't understand the color.

I used  Bb for trait 1 and Bg for parent 2. the squares said:

1. BB
2. bG
3. Bg
4. bg

It said for the color that

1. Brown
2. green
3. blue
4. green

How does that work? Some of the traits had nothing to do with the color.

### Colors show differences

Sofia - the square colors do not represent specific phenotype*s. They simply highlight the patterns. The two green squares does not mean that they have a green trait, itindicates that those two individuals have the same phenotype or genotype* (depending on the options selected).

need help!!!